SrasV1, Main, Exploration, bibRecord, 005A71

SARS coronavirus induces apoptosis in Vero E6 cells

Identifieur interne : 005A71 ( Main/Exploration ); précédent : 005A70; suivant : 005A72

SARS coronavirus induces apoptosis in Vero E6 cells

Auteurs : HUIMIN YAN [République populaire de Chine] ; GENGFU XIAO [République populaire de Chine] ; JIAMIN ZHANG [République populaire de Chine] ; YUANYANG HU [République populaire de Chine] ; FANG YUAN [Royaume-Uni] ; David K. Cole [Royaume-Uni] ; CONGYI ZHENG [République populaire de Chine] ; George F. Gao [Royaume-Uni]

Source :

Journal of medical virology [ 0146-6615 ] ; 2004.

RBID : Pascal:04-0417132

Descripteurs français

Pascal (Inist)
- Coronavirus, Apoptose, Mort cellulaire, Pathogénie, Pouvoir pathogène, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Apoptosis, Cell death, Coronavirus, Pathogenesis, Pathogenicity, Severe acute respiratory syndrome.

Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease. Its etiological agent has been convincingly identified as a new member of family Coronaviridae (SARS-CoV). It causes serious damage to the respiratory system yet the mechanism is not clear. Infection-induced apoptosis or necrosis is suspected but no direct evidence for this yet exists. To date, Vero E6 cells are the only cell line that could be used to replicate the virus with obvious CPE (cytopathic effect) in vitro. It is known for some viruses (including members of family Coronaviridae) that CPE can be caused either by virus-induced apoptosis (active death) or cell necrosis (passive death). In this study, we examined the apoptosis in the SARS-CoV infected Vero E6 cells. Indeed, the results do show that the CPE was induced by apoptosis rather than necrosis, shown by typical DNA fragmentation, through the existence of apoptotic bodies and swollen mitochondria. This observation has some implications for the SARS-CoV pathogenicity: SARS-CoV does induce apoptosis in cell cultures and might have the same effect in vivo, responsible for the severe damage of the respiratory system.</div>
</front>
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<name sortKey="Jiamin Zhang" sort="Jiamin Zhang" uniqKey="Jiamin Zhang" last="Jiamin Zhang">JIAMIN ZHANG</name>
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SARS coronavirus induces apoptosis in Vero E6 cells

SARS coronavirus induces apoptosis in Vero E6 cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri